大强度跳跃负荷下p38.MAPK对兔髌腱起止点微细结构影响

The Effect of p3 8.MAPK on the Microstructure of Rabbits Bone-Patellar Tendon Junctions under High-Intensity Jumping Load

DOI：10.12064/ssr.20210611

中文关键词:大强度跳跃负荷髌腱两端结合部 p3 8.MAPK

英文关键词:high-intensity jumping load bone-patellar tendon junction p38MAPK

基金项目:江苏省产学研合作项目(BY2019022)；江苏省体育局重大体育科研课题(ST192101)

作者	单位
陈一言	苏州大学体育学院,江苏苏州 215021
陆阿明	苏州大学体育学院,江苏苏州 215021
齐亚楠	北京体育大学,北京 100084
王琳	北京体育大学,北京 100084
刘云逸	北京体育大学,北京 100084

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中文摘要:

目的:使用电刺激定量跳跃装置使兔完成一次大强度跳跃运动,通过注射p3 8.MAPK抑制剂来研究p3 8.MAPK的活化作用在此种负荷下对兔髌骨髌腱结合部(PPTJ)与胫骨髌腱结合部(TPTJ)的产生的影响有何不同,是否会使组织结构分层相同的 2.个结合部之间产生差异。方法:1 4.只1 8.周龄雌性新西兰大白兔,随机分为对照组(CON,N=4)、跳跃组(J1,N=5)、跳跃后注射p3 8.抑制剂组(J 1.P,N=5)。每天15 0.次定量跳跃,训练结束2 4.h后处死取样,进行H&E与免疫组化处理,取样部位为PPTJ与TPTJ。结果:(1)J 1.P组PPTJ的IL- 6.含量明显大于CON组；(2)J 1.与J 1.P组PPTJ与TPTJ均有微细结构损伤,J 1.P组中 2.个部位之间的软骨带厚度有显著性差异；(3)CON组 2.个部位间IL-1β、IL- 6.有显著性差异,J 1.组PPTJ与TPTJ之间IL-1β、IL-6、TGF-β 1.有显著性差异,J 1.P组PPTJ与TPTJ之间IL-1β有显著性差异。结论:(1)一次大强度跳跃负荷会引起PPTJ与TPTJ微细结构变化(TPTJ的微细结构变化优于PPTJ)并使PPTJ与TPTJ之间TGF-β 1.差值显著增大,p3 8.MAPK被抑制后结局相反；(2)一次大强度跳跃负荷下,p3 8.MAPK的活化会维持PPTJ中IL- 6.含量的稳定,p3 8.MAPK被抑制使PPTJ与TPTJ之间IL- 6.含量显著性差异消失

英文摘要:

Objective: Using electrical stimulation quantitative jumping device for rabbits to complete a high-intensity jump training, injecting p38MAPK inhibitor to study the different activation of p38MAPK in rabbit patella patellar tendon junction (PPTJ) and tibial patellar tendon junction (TPTJ) under such load, and whether there will be a difference between two joints with the same hierarchical structure. Experimental methods: 14 18-week-old female New Zealand rabbits were randomly divided into control group (CON, N=4), jumping group (J1, N=5), and p38 inhibitor injection group after jumping (J1P, N=5). 24 hours after the training, the rabbits were sacrificed and sampled for H&E and immunohistochemical treatment. The sampling sites were patella patellar tendon junction (PPTJ) and tibial patellar tendon junction(TPTJ). Results: (1)The content of IL-6 in PPTJ of J1P group was significantly higher than that of con group; (2)Both PPTJ and TPTJ in J1 and J1P groups had microstructural damage, and there was significant difference in the thickness of cartilage zone between the two parts in J1P group; (3) There were significant differences in IL-1 β and IL-6 between the two parts of con group; there were significant differences in IL-1β, IL-6, TGF-β1 between PPTJ and TPTJ, and IL-1 β between PPTJ and TPTJ in J1P group. Conclusion: (1)A high-intensity jump load can cause the microstructural changes of PPTJ and TPTJ (the microstructural change of TPTJ is better than that of PPTJ), and the difference of TGF-β1 between PPTJ and TPTJ is significantly increased, and the result is opposite after p38MAPK is inhibited; (2)The activation of p38MAPK can maintain the stability of IL-6 content in PPTJ, and the inhibition of p38MAPK makes significant difference in IL-6 content between PPTJ and TPTJ disappeared.

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