末端病大鼠跟腱修复中胶原表达的研究

Researches on collagen express of achilles tendon repair of enthesiopathy of rats

DOI：

英文关键词:enthesiopathy Achilles tendon Collagen- I Collagen-Ⅲ

基金项目:江苏省社会发展项目（BS2006020）

作者	单位
张林	苏州大学体育学院
沈勇伟	苏州大学体育学院
齐红梅	南通大学体育科学学院

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中文摘要:

以雄性Wistar大鼠为实验材料，采用电刺激跳跃法建立末端病大鼠跟腱损伤模型，通过动态观察造模后末端病大鼠跟腱修复过程中胶原I和胶原III基因表达和蛋白含量的变化，探讨胶原在末端病大鼠跟腱修复中的作用及其机制。结果显示：（1）在末端病大鼠跟腱修复过程中胶原I在转录水平上的表达和蛋白含量的变化表现出先下降后升高的规律，表明胶原I在跟腱早期修复过程中被抑制。（2）胶原III在转录水平上的表达和蛋白含量均出现先升高后降低的变化特点，提示胶原III在跟腱修复的早期阶段起着十分重要的作用，这种作用在修复的前7 d效果非常明显。（3）末端病大鼠跟腱修复初期胶原I合成被抑制和胶原III含量的增加，表明胶原III能代偿被抑制合成的胶原I，积极参与肌腱的再生，直至胶原I代谢被激活后，两者共同促进肌腱的修复。

英文摘要:

With male Wistar rats as the subjects, the author adopted the method of electrical stimulation jumping to form a model of Achilles tendon enthesiopathy of rats. The Collagen-I and Collagen-III gene expression and the variation of protein content in the course of tendon repair of the rats with enthesiopathy were observed dynamically so as to explore the function and mechanism of collagen in tendon repair of rats with enthesiopathy. The result shows that in the course of repair, Collagen-ImRNA express and protein content decreased at first and then increased. This means that Collagen-I is restrained at the early stage of repair. The mRNA expression and protein synthesis of Collagen-III showed the characteristics of increasing at first and decreasing later. This means that Collagen-III plays a pivotal role in early-stage repair., especially in the first 7 days of repair. The restriction of Collagen-I synthesis and the increase of Collagen-III at the first stage of Achilles tendon repair indicate that Collagen-III may compensate synthesis-restrained Collagen-I and participate actively in regeneration of tendon until the activation of Collagen-I metabolism. Then they improve the repair of tendon together.

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