抗阻运动对增龄大鼠血脂及股四头肌相关基因表达的影响

Influence of Resistance Exercise on Blood Lipid and Gene Expression of Quadriceps of Aging Rats

DOI：

中文关键词:抗阻训练增龄大鼠血脂 AMPK mRNA PPARαmRNA PPARγmRNA

英文关键词:resistance exercise aging rats blood lipid AMPK mRNA PPARαmRNA PPARγmRNA

基金项目:

作者	单位
林文弢	广州体育学院
杜亚	广州体育学院
翁锡全	广州体育学院
范锦勤	韶关学院体育学院

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中文摘要:

目的：观察8周跑台抗阻运动对增龄大鼠血清血清游离脂肪酸（FFA）、血清甘油三酯（TG）、血清低密度脂蛋白（LDL）和血清高密度脂蛋白（HDL）及股四头肌AMPK、PPARα、PPARγ基因表达的影响。方法：40只SD大鼠自然增龄至18月龄后，随机分为安静组、0%、30%、50%和70%最大负重组进行8周的跑台抗阻运动，坡度35°，跑速15 m/min，隔天1次。用氧化酶法检测大鼠血清FFA、TG、LDL和HDL，用RT-PCR法检测大鼠股四头肌AMPK、PPARα和PPARγ的mRNA表达。结果：（1）与安静组和0%最大负重运动组相比，30%最大负重运动组的血清LDL下降均有统计学意义（P＜0.01，P＜0.05）；（2）与安静组和0%最大负重运动组相比，30%和50%最大负重运动组的股四头肌AMPK mRNA表达升高具有统计学意义（P＜0.05）；（3）与安静组和0%最大负重运动组相比，30%最大负重运动组的股四头肌PPARα mRNA表达升高具有统计学意义（P＜0.05）；（4）与安静组和0%最大负重运动组相比，30%最大负重运动组的股四头肌PPARγ mRNA表达升高具有统计学意义（P＜0.01），50%最大负重运动组的股四头肌PPARγ mRNA表达升高具有统计学意义（P＜0.05）。结论：8周跑台抗阻运动令运动组大鼠的血脂水平向有利于机体健康的方向变化；不同负重强度可影响血脂及相关因子的mRNA表达，以30%和50%的最大负重量较为合适；抗阻运动可能通过激活股四头肌AMPK而引起PPAR受体的基因表达。

英文摘要:

Objective: Observing the influence of 8-week resistance exercise on serum FFA, TG, LDL and HDL and the gene expression of AMPK, PPARαand PPARγof quadriceps of aging rats. Method: 40 SD rats, after reaching the age of 18 months, were randomly divided into the quiet group and the maximum load training groups of 0%, 30%, 50% and 70% for 8-week treadmill resistance exercise with the speed of 15m/min on a 35? slope once every two days. The serum FFA, TG, LDL and HDL were tested with the method of oxidase and the mRNA expression of AMPK, PPAR α and PPARγof quadriceps were detected with the method of RT-PCR. Result: (1) Compared with the quiet group and 0% maximum load group, LDL of 30% maximum load group decreased significantly (P < 0.01, P < 0.05); (2) Compared with the quiet group and 0% maximum load group, the expression of AMPK mRNA of 30% and 50% maximum load groups increased significantly (P < 0.05); (3) Compared with the quiet group and 0% maximum load group, the expression of PPARαmRNA of 30% maximum load group increased significantly (P < 0.05); (4) Compared with the quiet group and 0% maximum load group, the expression of PPARγmRNA of 30% and 50% maximum load groups increased significantly (P < 0.01, P < 0.05). Conclusion: The blood lipid level of the rats changes benignly after 8-week resistance exercise. Different load intensity can affect blood lipid and the mRNA expression of the related factors. The most suitable choice is 30% and 50% maximum load training. Resistance exercise may cause the gene expression of PPAR receptor by activating AMPK of quadriceps.

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