反复性力竭运动后大鼠心脏传导系统ADAMTS-1的变化及其在运动性心律失常发生中的作用

Changes of ADAMTS-1 of the Rat Cardiac Conduction System after Repeated Exhaustive Exercise and Its Role in Athletic Arrhythmia

DOI：

中文关键词:力竭运动金属蛋白酶-1 心脏传导系统

英文关键词:exhaustive exercise MMP-1 cardia conduction system

基金项目:国家体育总局体育科学研究所基本科研业务经费（10-01）

作者	单位
杨红霞	国家体育总局体育科学研究所
常芸

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中文摘要:

目的：探讨反复力竭运动后不同时相心脏窦房结、房室结和浦肯野氏纤维细胞炎性因子金属蛋白酶-1基因和蛋白水平的表达特点，为运动性心律失常发生机制的阐明提供实验依据。方法：100只健康成年雄性SD大鼠随机分为反复力竭组及相应的其对照组，每组10只。分别于力竭运动后0、4 h、12 h及24 h取材，进行心电图、免疫荧光组化及实时荧光定量PCR分析。应用激光显微切割技术定位并收集房室结和浦肯野氏纤维细胞研究细胞炎性因子ADAMTS-1的mRNA和蛋白表达的变化。结果：反复力竭运动后即刻心脏传导系统ADAMTS-1 mRNA和蛋白表达均出现不同程度升高，随后在4 h、12 h、24 h心脏传导系统窦房结、房室结、浦肯野氏纤维ADAMTS-1基因mRNA和蛋白表达下降（P＜0.05，P＜0.01），乃至恢复。心脏传导系统不同部位在反复力竭游泳运动后ADAMTS-1 mRNA和蛋白表达存有差异，其中，运动后即刻，浦肯野氏纤维ADAMTS-1蛋白表达显著低于窦房结和房室结（P＜0.01）。4 h，窦房结显著高于浦肯野氏纤维（P＜0.01）。24 h，窦房结显著低于房室结和浦肯野氏纤维（P＜0.01）。结论：反复力竭运动后即刻心脏传导系统ADAMTS-1 mRNA和蛋白表达均出现不同程度升高，作为炎性因子的大量表达，易引起传导系统炎性细胞浸润，细胞间质增殖乃至纤维化，是运动性心律失常的诱发因素之一。

英文摘要:

Objective To find out the expressive characteristics of sinoatrial node, atrioventricular node and Purkinje's fibre cell inflammatory factor MMP-1 gene and protein level at the different phases after repeated exhaustive exercises so as to provide experimental reference for the occurrence mechanism of athletic arrhythmia. Method 100 healthy adult male SD rats were divided randomly into repeated exhaustive exercise groups and control groups. There are 10 rats in each group. Samples were collected immediately, 4h, 12h and 24h after the exhaustive exercise for electrocardiogram, immunofluorescence technology and PCR analysis. Adopting microdissection technology to position and collect the changes of cardiac conduction system ADAMTS-1 mRNA and protein expression of atrioventricular node and Purkiny's fibre cell inflammatory factor ADAMTS-1. Result Cardiac conduction system ADAMTS-1 mRNA and protein expression immediately after repeated exhaustive exercise rose to some extent. Then the cardiac conduction system sinoatrial node, atrioventricular node and Purkinye's fibre ADAMTS-1 mRNA and protein expression decreased at 4h, 12h and 24 hours (P<0.05, P<0.01) until recovery. There is a difference between ADAMTS-1 mRNA and protein expression of the different parts of cardiac conduction system after repeated exhaustive exercise. Purkinye's fibre ADAMTS-1 protein expression was clearly lower than those of sinoatrial node and atrioventricular node immediately after the exercise (P<0.01). After 4 hours, protein expression of sinoatrial node was significantly higher than that of Puekinye's. After 24 hours, The protein expression of sinoatrial node was evidently lower than those of atrioventricular node and Purkinye's fibre (P<0.01). Conclusion ADAMTS-1 mRNA and protein expression rose to some extent immediately after repeated exhaustive exercise. Large quantity expression of inflammatory factor may cause inflammatory cell filtration , Intercellular substances proliferation and fibrosis. This is one of the risk factors for athletic arrhythmia.

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