一次性力竭运动后心肌核转录因子Kappa B的变化及其在运动性心肌微损伤发生中的作用

Changes of Myocardial Nuclear Transcription Factor Kappa B and It's Role in Athletic Myocardial Micro-damage after One-time Exhaustive Exercise

DOI：

中文关键词:力竭运动核转录因子KappaB(NF-κB) 心肌微损伤

英文关键词:sports, power constitution science & technology public service system culture

基金项目:国家体育总局体育科学研究所基本科研业务经费（10-01）

作者	单位
冀云肖	国家体育总局体育科学研究所
常芸

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中文摘要:

目的：研究一次性力竭运动后大鼠心肌NF-κB在基因与蛋白水平的分布及变化规律。方法：100只健康成年雄性SD大鼠，随机分为一次性力竭游泳运动组及安静对照组。应用RT-PCR和免疫荧光组化技术，从mRNA及蛋白水平研究大鼠心肌NF-κBp65的在力竭运动后不同时相的分布及表达变化。结果：对照组NF-κB主要分布于细胞质、细胞膜及血管内膜，偶见于细胞核内，力竭运动后NF-κB主要分布于细胞核、细胞膜和血管内膜。一次性力竭后6 h左心室NF-κB p65蛋白含量显著高于对照组、12 h组、24 h组（P＜0.05）；运动后即刻室间隔NF-κB p65蛋白含量显著高于对照组（P＜0.05），非常显著高于12 h组、24 h（P＜0.01），运动后6 h非常显著高于12 h组（P＜0.01）；运动后即刻右心室NF-κBp65蛋白含量即显著高于安静对照组、24 h组（P＜0.05），运动后6 h组显著高于安静对照组（P＜0.05）。一次性力竭运动后即刻心肌总的NF-κBp65蛋白含量显著高于对照组（P＜0.05），6 h组非常显著地高于对照组水平（P＜0.01），运动后12 h组显著低于即刻组和6 h组。一次性力竭即刻NF-κBp65mRNA含量显著高于一次性力竭24 h组（P＜0.05）；6 h显著高于对照组、24 h组（P＜0.05）；12 h显著高于24 h组（P＜0.05）。结论：一次力竭运动后不同时相心肌NF-κBmRNA和蛋白表达增加，可介导一系列炎症反应对细胞造成损伤,构成运动性心肌微损伤中发生机制之一。一次力竭运动后心脏各部位改变存有一定差异，其中室间隔及右心室NF-κB蛋白含量升高速度快,以室间隔NF-κB蛋白峰值水平最高,且右心室NF-κB蛋白改变恢复最慢。

英文摘要:

Objective To study the distribution and variation laws of rat heart muscle NF-κB in gene and protein level after one-time exhaustive exercise. Method 100 healthy adult male SD rats were divided randomly into one-time exhaustive swimming group and control group. RT-PCR and immunofluorescence technology were adopted to study distribution and expression variations of rat heart muscle NF-κB p65 at the different phases after exhaustive exercise from mRNA and protein level. Result In control group, NF-κB mainly distributed in cell plasma, cell membrane and tunic intima, sometimes in cell nucleus. After exhaustive exercise, NF-κB mainly distributed in cell plasma, cell membrane and tunic intima and cell nucleus. 6 hours after exhaustive exercise, NF-κB p65 protein level of left ventricle is obviously higher than those of the control group, 12h group and 24h group (P<0.05). The NF-κB p65 protein level of ventricular septum immediately after the exercise was significantly higher than that of the control group (P<0.05) and was much higher than the 12h group and 24h group (P<0.01). It was much higher than that of the 12h group 6 hours after the exercise. NF-κB p65 protein level of the right ventricle was distinctly higher than those of the control group and 24h group (P<0.05) and this level of the 6h group was evidently higher than that of the control group (P<0.05). The total NF-κB p65 protein level of heart muscle immediately after the one-time exhaustive exercise was significantly higher than that of the control group (P<0.05). This level of the 6h group was much higher than that of the control group (P<0.05). And the level of the 12h group was evidently lower than those of the immediate group and 6h group. NF-κB p65mRNA level immediately after the one-time exhaustive exercise was clearly higher than that of the 24h group in one-time exhaustive exercise (P<0.05). The level of the 6h group was obviously higher than those of the control group and 24h group (P<0.05). The level of the 12h group was significantly higher than that of the 24h group (P<0.05). Conclusion The increase of heart muscle NF-κBmRNA and protein expression at the different phases after the one-time exhaustive exercise may lead to a series of inflammatory reaction that can damage the cells. This is one of the occurrence mechanisms of athletic myocardial micro-damage. There is a difference in the changes of the different parts of heart after one-time exhaustive exercise. NF-κB protein levels of ventricular septum and right ventricle rise quickly. NF-κB protein level of the ventricular septum reaches the highest and the recovery of right ventricle NF-κB protein changes is the lowest.

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