力竭运动后不同时相大鼠心肌Egr-1蛋白的变化及其在运动性心肌微损伤发生中的作用

Changes of Egr-1 Protein and It's Functions in the occurrence of Athletic Myocardial Micro-damage at the Different Phases after Exhaustive Exercise

DOI：

中文关键词:力竭运动大鼠心肌早期生长应答基因-1（Egr-1）

英文关键词:exhaustive exercise rat myocardium early-stage growth response gene (Egr-1)

基金项目:国家体育总局体育科学研究所基本科研业务经费（09-10）

作者	单位
袁旭鑫	国家体育总局体育科学研究所
常芸

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中文摘要:

目的：探讨力竭运动后不同时相心肌早期生长应答基因Egr-1在蛋白水平的变化特点，为运动性心肌微损伤及心肌功能异常发生机制的阐释提供实验依据。方法：100只健康成年雄性SD大鼠，分为一次力竭游泳运动组、两周反复力竭游泳运动组及安静对照组，分别于力竭运动后即刻、6 h、12 h及24 h取材，应用免疫荧光组化技术和图像分析方法研究大鼠心肌Egr-1蛋白含量的变化。结果：一次力竭运动组大鼠心脏各部位Egr-1蛋白含量即刻组与对照组相比显著性升高（P＜ 0. 05），24 h组与对照组相比显著性降低（ P＜0. 05）；反复力竭运动组大鼠心脏各部位Egr-1蛋白含量即刻组与对照组相比显著性升高（P＜ 0. 05），6 h组、12 h组、24 h组大鼠心脏各部位Egr-1蛋白含量均显著低于对照组（P＜0. 05）。结论：不同力竭运动后心脏各部位Egr-1在运动后即刻明显升高，表明心肌细胞Egr-1对力竭运动刺激产生快速应激反应，可能诱导炎性细胞的趋化、聚集以及氧自由基的产生。而心脏各部位Egr-1在力竭运动后24 h内恢复到正常水平，说明力竭运动后心脏Egr-1改变属早期应激反应，且此反应属可复性改变。

英文摘要:

Objective To explore the variation characteristics of early growth response genes Egr-1 protein level so as to provide experimental reference for the occurrence mechanism of athletic myocardial micro-damage and myocardial dysfunction. Method 100 healthy adult male SD rats were divided into three groups of one-time exhaustive swimming group, two-week repeated exhaustive swimming group and no-exercise control group. Samples were collected immediately, 6 hours, 12 hours and 24 hours after exhaustive exercise. Immunofluorescence technology and image analysis were adopted to study the changes of rat myocardium Egr-1 protein level. Result The immediate Egr-1 protein levels of the different parts of rat myocardium increased significantly (P<0.05). Samples collected at 24 hours decreased distinctly compared to those of the control group (P<0.05). In repeated exhaustive group, the immediate Egr-1 protein levels of the different parts of rat myocardium increased significantly (P<0.05). Samples of Egr-1 protein levels of the different parts of rat myocardium at 6 hours, 12 hours and 24 hours were clearly lower than the control group (P<0.05). Conclusion Egr-1 of the different parts of heart increases immediately after the different exhaustive exercises shows that there is a rapid stress response of myocardial cell Egr-1 toward the stimulation of exhaustive exercise. This might lead to chemotaxis and aggregation of inflammatory cells and the production of OFR. Egr-1 of the different parts of heart returns normal level 24 hours after exhaustive exercise. This shows that Egr-1 after exhaustive exercise belongs to early-stage stress response and this response is reversible.

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