Objective: Observing the influence of 8-week resistance exercise on serum FFA, TG, LDL and HDL and the gene expression of AMPK, PPARαand PPARγof quadriceps of aging rats. Method: 40 SD rats, after reaching the age of 18 months, were randomly divided into the quiet group and the maximum load training groups of 0%, 30%, 50% and 70% for 8-week treadmill resistance exercise with the speed of 15m/min on a 35? slope once every two days. The serum FFA, TG, LDL and HDL were tested with the method of oxidase and the mRNA expression of AMPK, PPAR α and PPARγof quadriceps were detected with the method of RT-PCR. Result: (1) Compared with the quiet group and 0% maximum load group, LDL of 30% maximum load group decreased significantly (P < 0.01, P < 0.05); (2) Compared with the quiet group and 0% maximum load group, the expression of AMPK mRNA of 30% and 50% maximum load groups increased significantly (P < 0.05); (3) Compared with the quiet group and 0% maximum load group, the expression of PPARαmRNA of 30% maximum load group increased significantly (P < 0.05); (4) Compared with the quiet group and 0% maximum load group, the expression of PPARγmRNA of 30% and 50% maximum load groups increased significantly (P < 0.01, P < 0.05). Conclusion: The blood lipid level of the rats changes benignly after 8-week resistance exercise. Different load intensity can affect blood lipid and the mRNA expression of the related factors. The most suitable choice is 30% and 50% maximum load training. Resistance exercise may cause the gene expression of PPAR receptor by activating AMPK of quadriceps. |