微压氧对大强度训练大鼠氧化应激的影响

Effect of Mild-pressure Hyperbaric Therapy on the Oxidative Stress of the Rat Received High-intensity Exercise

投稿时间：2017-11-03

DOI：

英文关键词:mild-pressure hyperbaric therapy high intensity training oxidative stress

基金项目:国家体育总局体育科学研究所基本科研业务费资助项目（基本15-33）

作者	单位	E-mail
张冉	上海杉达学院	1519314332@qq.com
赵鹏	国家体育总局体育科学研究所	zhaopeng@ciss.cn

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中文摘要:

目的：围绕氧化应激中自由基的产生和清除来探讨微压氧对于大强度训练后的恢复机制，为微压氧在运动训练后恢复中的应用提供理论依据和实验支持。方法：8周龄雄性SD大鼠40只，经一周的适应性训练后筛选出30只，随机分为对照组（C组）、大强度训练后自然恢复组（HQ组，26.8 m/min，10°坡度）和微压氧恢复组（HM组），每组10只。本实验采用的微压氧仓氧浓度为26%左右，压强为3.6 PSI。训练周期为8周，每周训练5 d，每天1 h。HM组每次训练结束后即刻放入微压氧仓恢复1 h。在第8周最后一次训练结束24 h后进行麻醉，切取左侧腓肠肌用于氧化应激指标的检测和观察腓肠肌组织形态。结果：（1）与HQ组大鼠相比，HM组大鼠跑至力竭时间显著性长于HQ组（P＜0.05）。（2）HQ组肌肉MDA含量高于C组和HM组，具有显著性差异；HM组肌肉超氧化物歧化酶（SOD）活性显著性高于C组；HM组肌肉GSH-PX的活性高于C组和HQ组。（3）与C组相比，HQ组大鼠和HM组大鼠骨骼肌损伤轻微，但是HM组轻于HQ组。结论：大强度训练可造成大鼠骨骼肌氧化应激损伤，使其运动能力下降；而微压氧可提高骨骼肌抗氧化酶活性，减少骨骼肌脂质过氧化反应，提高运动能力。

英文摘要:

Objective: The purpose of this study,focusing on oxidative stress of the production and cleanup of free radical, is to investigate the recovery mechanism of mild-pressure hyperbaric therapy after high intensity training so as to provide theoretical basis and experiment support for the application of mild-pressure hyperbaric therapy to after-training recovery.Method: Thirty male SD rats (8 week old)were selected after the adaptability exercise from forty rats, and they were randomly divided into three groups: control group(C), quiet rest after high intensity exercise group(HQ, 28m/min,10°slope), mild-pressure hyperbaric therapy after high intensity exercise group(HM), with ten rats in each group. Oxygen concentration of mild-pressure hyperbaric therapy was about 26%, and the pressure was 3.6PSI. The rats did exercise for 1h/d, 5d/week and 8 weeks in total. The rats of HM immediately received mild-pressure hyperbaric therapy for 1h after the exercise. 24h after the last exercise in the 8th week, they were anesthetized and their left gastrocnemius was cut for the test of oxidative stress indicators and the observation of the tissue morphology of gastrocnemius.Result:（1）The time to exhaustion of HM group was significantly longer than that of HQ group(P＜0.05);（2）The MDA level of HQ group was significantly higher than those of C group and HM group(P＜0.05). SOD activity of HM group was significantly higher than that of C group(P＜0.05). GSH-PX activity of HM group was higher than those of C group and HQ group.（3）Compared to C group,the skeletal muscle injury of HM group and HQ group was slighter, and the injury of HM group was even slighter than that of HQ group.Conclusion: High intensity training can lead to skeletal muscle oxidative stress injury and result in poor sport performance. Mild-pressure hyperbaric therapy can enhance the activity of antioxidant enzyme of skeletal muscle, reduce the lipid peroxidation and therefore improve exercise capacity.

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