电刺激对过氧化氢诱导衰老肌管细胞能量代谢的影响
Effect of Electrical Stimulation on Energy Metabolism of Senescent Myoduct Cells Induced by Hydrogen Peroxide
投稿时间:2023-06-06  
DOI:10.12064/ssr.2023060601
中文关键词:电刺激  衰老肌管细胞  过氧化氢  能量代谢
英文关键词:electrical stimulation  C2C12 muscle tube  H2O2  energy metabolism
基金项目:辽宁省教育厅项目(L2014430);校高端科研成果培育资助项目(203070142207)
作者单位
苏艳红 辽宁师范大学 体育学院辽宁 大连 116029 
毕亮 辽宁师范大学 体育学院辽宁 大连 116029 
龚文翰 辽宁师范大学 体育学院辽宁 大连 116029 
宋涛 遵义医科大学 基础医学院贵州 遵义 563000 
李婷婷 辽宁师范大学 体育学院辽宁 大连 116029 
张世超 北京体育大学北京 100084 
摘要点击次数: 212
全文下载次数: 398
中文摘要:
      目的:运用电刺激肌管细胞收缩模拟骨骼肌收缩,并观察其对过氧化氢(H2O2)诱导的C2C12细胞衰老模型能量代谢相关指标表达的影响,以此研究体外肌管收缩对衰老骨骼肌细胞能量代谢影响的分子机制。方法:使用不同浓度的H2O2诱导肌管细胞建立衰老模型,并用β-半乳糖苷酶染色检测衰老水平;电刺激干预培育成功的衰老肌细胞,并分为对照组(C组)、H2O2诱导衰老组(H组)、对照+电刺激组(CE组)、H2O2诱导衰老+电刺激组(HE组)。各组细胞通过Western blot法对PGC-1α蛋白表达进行检测,ATP水平测试盒测量ATP水平。结果:经过浓度为100 μmol/L H2O2诱导肌管细胞24 h后成功构建骨骼肌细胞衰老模型。与C组比较,H组的ATP水平表达呈非常显著性的下降(P<0.01);CE组的ATP水平较C组呈非常显著性升高(P<0.01);HE组的ATP水平虽低于C组,但高于H组,且呈非常显著性差异(P<0.01);与C组相比,H组PGC-1α蛋白表达下降,呈非常显著性差异(P<0.01);HE组PGC-1α蛋白表达虽低于C组,但较H组呈非常显著性升高(P<0.01)。结论:H2O2是诱导骨骼肌肌管细胞衰老的一种有效方式,衰老肌管细胞的ATP和PGC-1α蛋白表达水平显著下降,进行电刺激干预后可以有效提高衰老肌管细胞ATP水平和PGC-1α蛋白表达。
英文摘要:
      Objective: Muscle tube cell contraction was simulated by electrical stimulation, and its effect on the expression of energy metabolism related indicators in C2C12 cell aging model induced by hydrogen peroxide (H2O2) was observed, so as to study the molecular mechanism of the effect of muscle tube contraction on energy metabolism of aging skeletal muscle cells in vitro. Methods: The senescence model was established by inducing myoduct cells with different concentrations of H2O2, and the senescence level was detected by SA-β-galactosidase staining. Electrical stimulation interfered with the successful growth of senescent muscle cells which were divided into control group (group C), H2O2-induced group (group H), control + electrical stimulation group (group CE), and H2O2-induced + electrical stimulation group (group HE). For cells in each group, PGC-1α protein expression was measured by Western blot and ATP level was measured by ATP level test kit. Results: Myotube cells were treated with 100 μmol/L H2O2 for 24 hours, and the aging model of skeletal muscle cells was successfully established. Compared with group C, ATP level in group H was significantly decreased(P<0.01); The ATP level in CE group was significantly higher than that in C group(P<0.01). The ATP level in HE group was lower than that in C group, but higher than that in H group, and the difference was very significant(P<0.01). Compared with group C, the expression of PGC-1α in group H was significantly decreased(P<0.01). Although the expression of PGC-1α in HE group was lower than that in C group, it was significantly higher than that in H group(P<0.01). Conclusion: H2O2 is an effective way to induce senescence of skeletal muscle myoduct cells. The protein expression levels of ATP and PGC-1α in senescent C2C12 cells decreased significantly. After electrical stimulation intervention, ATP level and PGC-1α protein expression in senescent C2C12 cells could be effectively increased.
查看全文  查看/发表评论  下载PDF阅读器
关闭