加压联合抗阻训练对T2DM大鼠骨骼肌胰岛素抵抗及质量的影响

Effects of Blood Flow Restriction Combined with Resistance Training on Insulin Resistance and Mass of Skeletal Muscle in T2DM Rats

投稿时间：2024-03-15

DOI：10.12064/ssr.2024031501

中文关键词:血流限制训练抗阻骨骼肌胰岛素抵抗

英文关键词:blood flow restriction training resistance skeletal muscle insulin resistance

基金项目:南京体育学院运动与健康工程协同创新项目（JSCIC-GP21007）；江苏省研究生科研与实践创新计划项目（KYCX23_2364）

作者	单位
查宇喆	南京体育学院运动健康学院，江苏南京 210014
喻乾	南京体育学院运动健康学院，江苏南京 210014
赵彦	南京体育学院运动健康学院，江苏南京 210014
刘怡然	南京体育学院运动健康学院，江苏南京 210014
林秋苹	南京体育学院运动健康学院，江苏南京 210014

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中文摘要:

目的：探讨加压联合低强度抗阻训练对2型糖尿病（T2DM）大鼠糖代谢、骨骼肌胰岛素抵抗（PI3K/Akt/Glut4）通路及其下游P70S6K蛋白表达和质量的影响。方法：HFD饲料喂养8周，联合注射35mg/kg 链脲佐菌素构建T2DM大鼠模型，造模成功大鼠均分为5组（n=9），2型糖尿病对照组（DR）：不进行运动干预；单纯加压训练组（BRT）：加压5 min休息5 min，4次循环；高强度抗阻训练组（HRT）：50%~75%1RM；低强度抗阻训练组（LRT）：35%~55%1RM；加压联合低强度抗阻训练组（BLRT）：加压联合35%~55%1RM。运动后测糖代谢指标，Western blot法测PI3K/Akt/Glut4通路及P70S6K相关蛋白表达。结果：①8周后，BRT组、LRT组、BLRT组空腹血糖显著下降（P＜0.05）。②与DR组比较，HRT组p-PI3K、p-Akt和Glut4显著增加（P＜0.01）；与BRT组比较，HRT组p-PI3K、Glut4显著增加（P＜0.05）。③与DR组比较，BLRT组p-Akt、Akt/p-Akt和Glut4显著增加（P＜0.01）；与BRT组比较，BLRT组Glut4表达显著增加（P＜0.01）；与HRT组比较，BLRT组Akt/p-Akt比值显著增加（P＜0.05）。④BLRT组和HRT组p-Akt、Glut4、P70S6K、p-P70S6K蛋白表达和p-P70S6K/P70S6K比值相近。结论：加压联合低强度抗阻训练能够增加p-Akt/Akt的比值，上调Glut4表达，改善T2DM大鼠骨骼肌胰岛素抵抗，降低血糖。加压联合抗阻训练对骨骼肌质量无显著影响。

英文摘要:

Objective: To investigate the effects of blood flow restriction combined with low-intensity resistance training on glucose metabolism, skeletal muscle insulin resistance (PI3K/Akt/Glut4) pathway and its downstream P70S6K protein expression and mass in T2DM rats. Methods: The type Ⅱ diabetes mellitus rat model was constructed by feeding high-sugar and high-fat diet for 8 weeks and injecting 35 mg/kg streptozotocin. The successfully modeled type Ⅱ diabetes mellitus rats were divided into 5 groups (n=9). Type Ⅱ diabetes control group (DR): no exercise intervention; pure blood flow restriction training group (BRT): blood flow restriction for 5 minutes, rest for 5 minutes, 4 cycles; high-intensity resistance training group (HRT): 50%~75% 1RM; low-intensity resistance training group (LRT): 35%~55% 1RM; blood flow restriction combined with low-intensity resistance training group (BLRT): blood flow restriction combined with 35%~55% 1RM. After exercise, glucose metabolism indexes were measured, and the expression of PI3K/Akt/Glut4 pathway and P70S6K-related proteins were measured by Western blot. Results: ①After 8 weeks, fasting blood glucose in BRT, LRT and BLRT groups decreased significantly (P＜0.05). ②Compared with DR group, p-PI3K, p-Akt and Glut4 were significantly increased in HRT group (P＜0.01). Compared with BRT, p-PI3K and Glut4 in HRT group were significantly increased (P＜0.05). ③Compared with DR group, p-Akt, Akt/p-Akt and Glut4 were significantly increased in BLRT group (P＜0.01). Compared with BRT, Glut4 expression in BLRT group was significantly increased (P＜0.01). Compared with HRT, the Akt/p-Akt ratio in BLRT group was significantly increased (P＜0.05). ④The expression of p-Akt, Glut4, P70S6K, p-P70S6K protein and the ratio of p-P70S6K/P70S6K were similar in BLRT and HRT groups. Conclusion:Blood flow restriction combined with low-intensity resistance training increases the p-Akt/Akt ratio, upregulates Glut4 expression, and attenuates skeletal muscle insulin resistance in T2DM rats, thereby reducing blood glucose.Blood flow restriction combined with resistance training had no significant effect on skeletal muscle mass.

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