持续游泳训练模式下大鼠脑部miRNA的深度测序及其调控功能分析

Deep Sequencing and Regulatory Functional Analysis of miRNA in Rat Brain Under Continuous Swimming Training Mode

投稿时间：2024-04-09

DOI：10.12064/ssr.2024040902

英文关键词:continuous training brain deep sequencing target gene regulatory function

基金项目:国家自然科学基金项目（31540030）

作者	单位
张瑞萍	烟台大学体育学院，山东烟台 264005
赵彦宏	鲁东大学农学院，山东烟台 264025
张安民	烟台大学体育学院，山东烟台 264005

摘要点击次数: 0

全文下载次数: 2

中文摘要:

目的：拟鉴定出持续游泳训练模式刺激下大鼠脑部差异表达的miRNA，从miRNA调控基因表达的角度进一步分析持续有氧游泳运动影响脑部功能的遗传应答机制。方法：将124只大鼠随机均分为持续游泳训练组（CST）和对照组（NC），建立大鼠持续游泳模型。分别构建两组大鼠脑部组织sRNA文库，通过Illumina Solexa测序平台进行高通量测序，使用EXPR_SIG 3.0程序对miRNA的表达进行差异性分析。采用实时荧光定量PCR（qRT-PCR）法检测两组组织中有显著差异的miRNA的表达水平。结果：两组样品共筛选出2 419个已知miRNA，新预测miRNA共114个。与NC组相比，CST组鉴定出512个响应持续游泳训练而差异表达的miRNA，其中397个表达显著上调，115个表达显著下调（P＜0.005）。通过靶基因预测发现，差异表达的miRNA参与了脑部362个基因的表达调控。结论：持续有氧训练可使大鼠脑部miR-379-5p、miR-139-3p、miR-125b-5p、miR-30e、miR-128、miR-101等多个miRNA出现显著差异性表达，差异表达的miRNA靶基因可涉及脑细胞的多种代谢调节功能，但具体调控机制及其在疾病防治中的作用还有待进一步验证。

英文摘要:

Objective: To identify the differentially expressed miRNA in rat brain stimulated by continuous swimming training mode, predict their target genes and explore their potential regulatory functions. Methods:124 rats were randomly divided into continuous swimming training group (CST) and control group (NC) ,and the model of continuous swimming was established. After constructing the sRNA libraries of two groups of rat brain tissues respectively, high-throughput sequencing was carried out through Illumina Solexa sequencing platform, and the difference of miRNA was analyzed by EXPR_SIG 3.0 program. Real-time fluorescent quantitative PCR (qrt-PCR) was used to detect the significant difference in Mirna expression between the two groups. Results: A total of 2 419 known miRNA were screened out and 114 new ones were predicted. Compared with NC, 512 differentially expressed miRNA in response to continuous swimming training were identified in CST group, of which 397 were significantly up-regulated and 115 down regulated（P＜0.005）.The target gene prediction showed that differentially expressed miRNA were involved in the expression regulation of 362 genes in the brain. Conclusion: Continuous aerobic training could significantly differentially express miR-379-5p, miR-139-3p, miR-125b-5p, miR-30e, miR-128, miR-101 and other miRNAs in the brain of rats, and the differentially expressed miRNA target genes were involved in various metabolic regulatory functions of brain cells. However, the specific regulatory mechanism and its role in disease prevention and treatment need to be further verified.

查看全文查看/发表评论下载PDF阅读器

关闭