|
| 再竭运动恢复期大鼠纹状体微透析液中Glu和GABA含量的变化 |
| Changes of Glu and GABA in the striatum of rat brain in the recovery period after exhaustive exercise |
| |
| DOI： |
| 中文关键词:微透析 纹状体 运动性中枢疲劳 Glu GABA |
| 英文关键词:micro-dialysis striatum exercise-induced central fatigue Glu GABA |
| 基金项目:江苏省科技厅自然科学基金项目（BK2011863） |
|
| Hits: 3474 |
| Download times: 2724 |
| 中文摘要: |
| 目的：观察大鼠力竭运动恢复期纹状体细胞外液谷氨酸（Glu）、γ-氨基丁酸（GABA）含量的动态变化，分析它们的变化特点与相互关系，为探索运动性中枢疲劳产生和恢复机制提供实验依据。方法：将12只SD雄性大鼠随机分为安静对照组（6只）和力竭运动组（6只），于运动后4 h、5 h、 6 h、 8 h、24 h收集微透析样品并采用毛细管电泳-激光诱导荧光法直接进样检测。结果：力竭运动恢复期大鼠纹状体细胞外液中Glu含量高于安静水平，其恢复期变化呈先上升后下降的趋势，运动后8 h达峰值，运动后24 h仍显著高于安静水平（P﹤0.05）；GABA含量高于安静水平，其恢复期变化呈先上升后下降的趋势，运动后6 h达峰值，运动后24 h仍显著高于安静水平（P﹤0.05）；Glu/GABA比值显著低于安静水平（P﹤0.05），运动后24 h仍显著低于安静水平（P﹤0.05）。结论：运动性中枢疲劳产生时，机体同时促进Glu、GABA的释放，但是GABA释放量更高，抑制性神经递质释放占优势，通过直接通路和间接通路抑制运动。恢复期后期两者均逐渐下降，纹状体兴奋性逐渐升高，疲劳得以逐渐恢复。力竭运动恢复期大鼠纹状体细胞外液Glu、GABA含量升高及恢复均有延迟性，力竭运动后24 h恢复仍未完成；大鼠纹状体细胞外Glu/GABA显著低于安静水平，24 h恢复期抑制性神经递质仍起主导作用。 |
| 英文摘要: |
| To observe the changes of extracellular glutamate(Glu)and γ-amino-butyric acid(GABA) concentrations after exhaustive exercise, analyze their characteristics and interrelations and provide experimental reference for the occurrence and recovery mechanism of exercise-induced central fatigue. Method: 12 SD male rats were randomly divided into quiet control group(6) and exhaustive exercise group(6). The micro-dialysis samples were collected at 4h, 5h, 6h, 8h and 24 hours after the exercise. The samples were determined by HPCE-LIF. Results: The concentration of Glu of the Exhaustive Exercise group in the recovery period was higher than that of the control group. The changes in the recovery period showed the tendency of increasing first and decreasing later. The peak value was reached at 6 hours after the exercise, and at 24 hours after the exercise, the concentration of Glu was still higher than that of the control group(P<0.05). GABA of the exercise group was higher than that of the control group and the changes in the recovery period showed the tendency of increasing first and decreasing later. The peak value was reached at 6 hours after the exercise, and at 24 hours after the exercise, GABA was still higher than that of the control group(P<0.05). Glu/GABA ratio of the exercise group was significantly lower than that of the control group and was still evidently lower than that of the control group at 24 hours after the exercise(P<0.05). Conclusion: When central fatigue is induced by exercise, Glu and GABA are stimulated to release at the same time and more GABA is released. The release of inhibitory neurotransmitter prevails and suppresses physical performance through direct and indirect paths. Both of them decrease in the latter period of recovery and the excitation of striatum gradually increases to restore fatigue. The delay of the increase of Glu and GABA and recovery can be observed in the recovery period after exhaustive exercise. Recovery cannot be completed at 24 hours after exercise. Extracellular Glu / GABA levels are significantly lower than those in the state of quietness. Inhibitory neurotransmitter still plays a leading role in the 24-hour recovery period. |
| View Full Text View/Add Comment Download PDF reader |
| Close |
